While I usually appreciate ability of ListView to adjust column widths, there are situations when I want it fixed to certain size.

For this occasion I already had column size fixed:

column.Width = listView.ClientRectangle.Width - SystemInformation.VerticalScrollBarWidth;

One would think that cancelling ColumnWidthChanging would be sufficient, e.g.:

private void listView_ColumnWidthChanging(object sender, ColumnWidthChangingEventArgs e) {
    e.Cancel = true;
}

However, this will not work. Column width will stay fixed while you are moving mouse around only to change once you release the mouse button. To cancel column width, we need to reset the size too:

private void listView_ColumnWidthChanging(object sender, ColumnWidthChangingEventArgs e) {
    e.Cancel = true;
    e.NewWidth = listView.Columns[e.ColumnIndex].Width;
}

A bit more code then I would personally expect, but easy enough.